Forensic Phycology plays a very important role in drowning cases where the site of drowning or the cause of death is suspected. Diatom profiles of various water bodies have been formulated by different researchers for different region for the same purpose. Apart from this, it also helps in analyzing the water quality index and diatom specificity of the region. The main aim of this study is to evaluate the diatoms of the major water bodies of tourist attractions sites of Rajasthan, which are more susceptible to drowning accidents. Ana Sagar, Fateh Sagar, Foy Sagar, Jaisamand Lake, Pichola Lake, Nakki Lake and Sambhar Lake were selected for this study. This study also aims to identify the site- specific diatoms and commonly occurring diatoms. The samples were collected during January-March and further analysis was done by centrifugation and slide preparation. The results of the qualitative estimation showed the presence of Nitzschia and Aulacoseira as commonly occurring diatom whereas Navicula was rarely seen. Two different species of Cyclotella were also observed like Cyclotella ocellata, Cyclotella menghiniana. Encyonopsis and Encyonema were the species which were reported for the first time in Rajasthan. Only Meloseira was reported in the Sambhar, the largest saline lake.