Our eco-friendly approach aims to provide solutions that not only advance scientific knowledge but also address the need for sustainable nanoparticle synthesis. This green synthesis strategy will address relevant social challenges by reducing the impact on the environment, reducing the use of dangerous chemicals, aligning with social ideals, and many other ways. In the present study, Carica papaya leaf extract acts as a reducing and capping agent for the synthesis of silver nanoparticles. Once the synthesis process is completed, the antibacterial activity of silver nanoparticles is tested against gram positive and gram-negative Escherichia coli, Staphylococcus aureus, and Aspergillus niger as well. This test involves observing the formation of a clear zone around nanoparticles, indicating the inhibitory effect on specific microorganisms. Additionally, HPLC (High Performance Liquid Chromatography) was employed to assess the purity and concentration of silver nanoparticles, offering a detailed analysis of their characteristics. Following characterization, the nanoparticles are characterized using UV spectroscopy. The optical density values gradually increase, the absorbance peaks were seen at 410 and 640nm for Carica papaya indicating successful nanoparticle production. Then FTIR (Fourier Transform Infrared Spectroscopy) was performed to determine the functional groups present in our silver nanoparticles. These peaks indicate the presence of aliphatic primary amine, carbodiimide, and alkene functional groups. To study shape and size, nanoparticles were seen under a scanning electron microscope (SEM). The size of nanoparticle between 50 to 500 nm. Research on the potential uses of these nanoparticles is ongoing while keeping all of these considerations in mind.