Introduction: Maxillary canine is most important tooth in the dental and facial esthetics because of the amount of visible coronal structure. It has wider pulp chamber, single straight root and withstand with high masticatory forces so its survival rate is high. Objective: The objective of the current study is to assess the effect of age and gender on dimension of pulp to tooth crown ratio in permanent maxillary canine by using CBCT in subjects visiting Khyber College of Dentistry Peshawar. Material and Methods: It’s a cross-sectional study in which 180 subjects of both gender fulfill the inclusion criteria were selected and there age range from 13 years to 60 years. Results: The mean age was 27.23±10.5 (SD). The high frequency was in 13-23 years and lowest frequency was in 54 and above. As age is compared with variables, it shows that P value is highly significant (p=.005). As gender is compared with tooth variables it also shows that P-value is highly significant (p=.005) Conclusion: The relationship between age and pulp chamber volume was significant (p=.005) which demonstrated that with age the size of the pulp chamber decreases.