Biomedical waste, sometimes known as hospital trash, is created during the treatment of people as well as during biologics research, that contains potentially contagious materials and affect the environment due to improper disposal of waste. As nurses are backbone of the health care delivery system though they are working 24 hours a day. Novice nursing students have less knowledge on impact of biomedical waste on environment& its prevention. The current Pre-experimental one group pre-test post-test research study conducted in 3 nursing colleges of Odisha with 500 first year nursing students who are selected randomly by using convenience sampling technique. The result of the current study revealed that, majority of nursing students in Post-test (77.33%) are having good knowledge than Pre-test knowledge (3.33%).There was a significant mean difference (7.1) exists between pre-post test knowledge score and 0.1 SD difference exists between the pre and post-test. The present study revealed that, there is lack of adequate knowledge among nursing students regarding management of biomedical waste in view of its impact on the different aspects of the environment. The management of biomedical waste should be assessed as soon as possible in order to raise awareness for proper collection, segregation and disposal to prevent contamination of waste towards environment amongfuture nursing officers at their training courses.