Context: The physical and psychological advantages of yoga are well known. Yogic practice is gaining popularity these days due to the fact that it trains and disciplines the mind. Yogic philosophy claims the ability to transport an individual into a state of perfect psychological and physical peace. This present study was focused on evaluating the effect of yogic practice on Mental toughness and Anxiety. Aim: The study was conducted to investigate the Effect of Yogic Practice on Mental Toughness and Anxiety of Physical Education Students. Methods and Material: Forty (40) male students from Manipur University's Department of Physical Education and Sports Science were randomly selected as study participants in order to fulfill the purpose of the research. They were further divided into two groups, the Experiment Group (EG) and Control Group (CG), each with 20 participants. The subjects ranged in age from 18 to 23 years old. The eight-week Yogic practice consists of five days a week, with one hour of sessions in the morning (6:00am to 7:00 am). The yoga practice was performed by the experiment group, while no particular yoga practice was performed by the control group. The pre- and post -tests were conducted before and after the eight weeks yogic practice by using mental toughness questionnaire (MTQ) by Alan Goldberg and sport competition anxiety test (SCAT) by Rainer Martens. Results: The data was examined statistically using a paired sample t-test, and the level of significance was fixed at 0.05. The results of the present study revealed that the eight weeks of yogic practice had a significant influence on the sports competition anxiety and mental toughness of the experiment group (EG) compared to the control group (CG).