Estimation of burn injury age and vitality both in living and dead is essential and pivotal issue in forensic pathology to evaluate accurately its causal relationship to death. Nitric oxide and interleukin-6 (IL-6) play an important role in skin burn healing. This study shows that afterburn injury, there was a strong increase inflammatory infiltrates in the 2nd and 3rdweekante-mortem burn and decrease in post-mortem burn. Our study demonstrated, there was a statistically significant difference in both iNOS and IL-6 expression between the different time periods of the ante-mortem burn and decreased remarkably in the post-mortem burn. The expression of iNOS and IL-6 proteins in skin burn injury healing in patients by immunohistochemistry for its forensic application in determination of skin burn age and vitality in addition to their possible role in differentiating between ante-mortem and post mortem burn was identified. Further research on various burn types is recommended.