Because of their capacity to lower reactive oxygen species formation and hence prevent various age related illnesses, characterization of antioxidant-active chemical is of significant interest. Antioxidants have the capacity to protect organisms from oxidative stress generated by free radicals. A lot of study is being done throughout the world to uncover natural antioxidants in various herbal origin medications. The reported study include phyto-chemical screening, determination of poly-phenolic content and antioxidant activity using different in vitro models of the hydro-methanolic fraction of Ceiba pentandra Linn. bark. The anti-oxidant activity was scrutinized spectrophotometrically. Phyto-chemical screening revealed the presence of Phenolics, Tannins, Saponins, Flavonoids, Steroids and Carbohydrates. The total phenolic content and total flavonoid content was found to be 239.11 ± 0.769 mg GAE/gm dry extract and 201.66 ± 1.154 mg RTE/gm dry extract respectively. In DPPH scavenging activity percentage inhibition was found to be in the range of 25.64 to 73.69 for extract of Ceiba pentandra Linn. with IC50 value 141.74 μg/ml and in Ferric Reducing Power Assay extract showed dose dependent increase in absorbance indicating good antioxidant activity. Our findings show that hydro-methanolic extract of Ceiba pentandra Linn. is a possible source of natural antioxidants, which validates its use in folkloric medicine.