Phytochemicals can help prevent or treat numerous common illnesses. Identifying and isolating these phytochemicals would greatly benefit human civilization. This study aims to evaluate and compare the antioxidant activity and phytochemical profiles of hexane, chloroform, ethyl acetate, and water extracts of the whole plant Ouret lanata. The dried plant powder was sequentially extracted with ethanol for eight hours, and the solvent was evaporated under reduced pressure. Similarly, the air-dried powder was extracted with water, and the water was evaporated. This research investigates the phytochemicals and performs both qualitative and quantitative evaluations. Tannin was the only substance detected in all extracts. Tests for alkaloids, flavonoids, and tannins were positive in the chloroform extract. Flavonoids, glycosides, and tannins were significantly more abundant in all three extracts. The aqueous extract tested positive for tannins and flavonoids. According to the results of TLC for phenol and flavonoid analysis, the O. lanata extract contains additional classes of phenols and flavonoids compared to the standard used for comparison. The methanolic extract of O. lanata was found to have a total phenolic content of 282.86 ± 0.52 mg/g, while the hexane, chloroform, and ethyl acetate extracts had phenolic contents of 81.25 ± 0.81, 219.12 ± 0.17, and 161.42 ± 0.34 mg/g, respectively. The total flavonoid content was found to be 145.3 ± 2.13, 163.4 ± 1.42, 88.2 ± 2.61, and 123.7 ± 2.41 mg quercetin/g, indicating a high flavonoid content. The O. lanata extract showed high antioxidant and antimicrobial potential. The inhibition of cytotoxic effects was determined using the Human Keratinocyte Cell Line, HaCaT.