Objective: To Assess the protective effects of various fractions of Chlorophytum borivilianum L. root extract against doxorubicin-induced cardiotoxicity in albino rats. Methods: Adult albino rats were divided into seven groups: control, doxorubicin (DOX), DOX + Vit.E, DOX + Petroleum ether CB fraction, ethyl acetate CB fraction, Chloroform CB fraction & aqueous CB fraction. Cardiotoxicity was induced by administering DOX (15 mg/kg, i.p) over a 2-week period. Post-treatment, biochemical markers are (CK-MB, LDH, and cardiac troponin I) &oxidative stress parameters (MDA, SOD, and catalase) & ECG were measured. Histopathological examinations of heart tissues were conducted to assess structural changes. Results: Doxorubicin significantly increased serum cardiac markers and oxidative stress levels, indicating cardiotoxicity. Treatment with Chlorophytum borivilianum fractions, particularly the ethyl acetate CB fraction, significantly mitigated these alterations. The ethyl acetate Chlorophytum borivilianum (CB) group exhibited the most notable cardioprotective effects, evidenced by the normalization of biochemical parameters and improved histopathological profiles, compared to DOX group only. Conclusion: Chlorophytum borivilianum root fractions, especially its ethyl acetate fraction, demonstrates significant cardioprotective effects against DOX-induced cardiotoxicity. This protective effect is likely mediated through its antioxidant properties, suggesting its potential as a therapeutic agent for managing chemotherapy-induced cardiac damage.