Dental stem cells, derived from the tooth structure, are the matured stem cells which have attracted the researchers attention in the last decade. Knowledge in this field is being developed rapidly and having insight in this field among the experts seems essential. The purpose of this study was to investigate the awareness, knowledge and attitude of dental specialists in Gorgan regarding the use of dental stem cells in dentistry. The current research is a cross-sectional descriptive study that was conducted based on the design of Nourbakhsh et al. questionnaire with closed questions among 60 dental specialists in Gorgan. The obtained data were analyzed with Mann-Whitney, t-test and ANOVA tests and a significance level of 0.05 was considered. The general sample included 60 specialist dentists working in Gorgan. The average age in the whole study was 39.8 years. The average score of awareness (based on 5-0) was 3.38±0.84, the average score of knowledge (based on 11-0) was 2.6±1.58, and the average score of attitude (based on 60-14) was 47/51±4.37. The results showed that age, sex, work experience, field of expertise and activity site had no significant relationship with the awareness, knowledge and attitude of the participants The obtained data revealed that despite relatively high awareness and positive attitude, the knowledge about the use of stem cells is weak.