Cassia auriculata fractions and its isolated novel compound dianthrone A and B were subjected to in vitro antioxidant and antibacterial activity against pathogenic bacteria. The results elucidated that the methanol fraction and the isolated compounds demonstrated dose dependent antioxidant activity when tested with DPPH with an inhibition of 3-10 % at 2.5 mg/ml concentration and displays highest inhibition at 20mg/ml with 26-82%. In FRAP, the highest reducing ability was found at 31-78% at 20 mg/ml concentration. The results of enzymatic antioxidant such as SOD (38.42, 32.64 and 28.42 units); catalase with 46.34, 47.44 and 39.62µmol/min mL; GPX with 89.16, 92.14 and 90.66 was observed for Cassia auriculata fractions and dianthrone A & B. Similarly, methanolic fraction and the isolated compound dianthrone A and B revealed maximum zone of inhibition with 23.39 mm against Streptococcus mutans whereas, the isolated compounds demonstrated significant activity with an average of 11-22 mm zone against tested species at the concentration of 200µg/mL. Protein denaturation study revealed Cassia auriculata fractions, dianthrone A and B treatment inhibits protein denaturation there by reducing the effect of arthritis and inflammation. The current study corroborates our previous research findings exploring the potential of Cassia auriculata a promising remedy for various ailments.