This study focused on the in-vitro biological activities of ethanolic extracts of Phillyrea latifolia, Phillyrea angustifolia and spirulina. Belonging to the Oleaceae family. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. In addition, in vitro anti-diabetic activity was achieved by inhibition of the α-glucosidase enzyme. Extraction of the ethanolic extracts of Phillyrea latifolia, Phillyrea angustifolia and Spirulina gave yields of 0.89%, 0.93% and 11.18% respectively. The tests showed that the ethanolic extracts of these two plants, as well as spirulina, possessed significant antioxidant potential, with IC50s of 36 μg/ml, 30.85 μg/ml, and 25.76 μg/ml respectively. In addition, the ethanolic extracts of the two plants and spirulina showed significant anti-diabetic potential, with IC50s of around 22.19 mg/ml, 24.55ug/ml and 18.87ug/ml. These results highlight the antioxidant and anti diabetic potential of ethanolic extracts of Phillyrea latifolia, Phillyrea angustifolia and spirulina, which is known to be a natural antioxidant. The results obtained confirm the information already available in the scientific literature, underlining the interest of these extracts as promising natural agents for improving health and well-being. These extracts could offer effective natural alternatives for preventing or treating diseases linked to oxidative stress and diabetes, reinforcing their relevance in the field of natural medicine and dietary supplements.