This study aimed to evaluate the phytochemical composition of Prunella vulgaris extracts obtained using different solvents and quantify specific bioactive compounds present in each extract. Phytochemical screening revealed varying degrees of presence or absence of alkaloids, carbohydrates, glycosides, saponins, phenols, tannins, flavonoids, proteins, and diterpenes across chloroform, ethyl acetate, ethanolic, and aqueous extracts. Chloroform and ethyl acetate extracts showed predominantly negative results for alkaloids and other constituents, indicating a lower affinity for polar compounds like phenols, flavonoids, and alkaloids. In contrast, ethanolic and aqueous extracts exhibited a broader spectrum of phytochemicals. Ethanolic extract notably tested positive for flavonoids, phenols, proteins, and moderate alkaloid content, highlighting ethanol's effectiveness in extracting these compounds. Quantitative analysis confirmed that the ethanolic extract had the highest concentrations of total flavonoids (0.699 mg/100 mg), total phenols (0.565 mg/100 mg), and total alkaloids (0.245 mg/100 mg) among all tested extracts, suggesting ethanol as a suitable solvent for extracting diverse bioactive compounds from Prunella vulgaris. These findings underscore the potential medicinal uses of Prunella vulgaris extracts and suggest that solvent selection influences phytochemical composition and therapeutic properties.