Next-generation sequencing (NGS) development in forensic DNA investigation constitutes a major technological breakthrough in crime scene examination by acquiring high-resolution genetic data. This study intends to assess the extent to which NGS is helpful in comprehensive profiling in comparison with the existing profiling tools in forensic investigations. Of the crime scene DNA specimens, a sample amounting to n=150 was analyzed using NGS involving autosomal STRs, SNPs, and mtDNA. There were statistically significant increases in profile completeness (p<0.05) with a difference prevailing in disadvantaged and mixed samples. NGS increased 35% in informative loci reducing the partial matches in ambiguity. Because SNPs and mtDNA are included in NGS panels, individualization estimates in a complex mixture were advanced achieving 99.2% discrimination power. The research results offer convincing arguments for future practice and integration of NGS techniques which could deal with limitations posed by traditional methods. This research helps to fill the gaps in forensics identification by addressing cold case searches and disaster victim identification utilizing NGS based sequencing methodologies.