Thuja orientalis, Punica granatum and Aloe barbadensis miller are well known traditional medicinal herbs for antifungal activity. The combination of these herbs has not been studied so far for its activity against vaginitis. Hence, the present study was designed to evaluate anti-fungal activity of polyherbal gel formulations for vaginal infection with a combination of ingredients such as Thuja orientalis, Punica granatum, Aloe barbadensis miller and Coconut oil. In this present study, polyherbal formulation was prepared using methanolic extracts of Thuja orientalis and Punica granatum, fresh leaves of Aloe barbadensis miller and Coconut oil by incorporating with Carbopol 934 as a gelling agent. The prepared polyherbal formulations were evaluated with various parameters which includes organoleptic, pH, extrudability, Spreadbility, homogeneity, grittiness and stability test. All these different formulations were screened for anti-fungal activity against Candida albicans. The results of this study reveal that, the evaluation parameters and stability studies for all the four formulations were observed and found to be satisfactory. The F4 formulation exhibited highest inhibitory effect against C. albicans. Whereas F3 and F2 formulations showed similar or moderate inhibitory activity. This is the first report on the scientific evaluation of Thuja orientalis, Punica granatum, Aloe barbadensis miller and Coconut oil combinations as polyherbal gel formulations for anti-fungal activity as vaginal-care.