Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production or action. Conventional insulin therapy, primarily administered via subcutaneous injection, poses challenges such as patient discomfort, needle phobia, and poor compliance. This study aims to develop and evaluate a novel insulin gel formulation for transdermal delivery, offering a non-invasive alternative to injections. The insulin gel was formulated using biocompatible polymers that enhance skin permeability and ensure controlled release. The gel's physicochemical properties, including viscosity, pH, and drug content, were characterized to ensure stability and efficacy. In vitro skin permeation studies were conducted using Franz diffusion cells to assess the transdermal delivery efficiency of insulin. Pharmacodynamic evaluations in diabetic animal models demonstrated the gel's ability to lower blood glucose levels effectively, comparable to traditional injection methods. The study also examined the gel's safety profile, observing no significant skin irritation or adverse reactions. Overall, the insulin gel formulation presents a promising transdermal delivery system, potentially improving patient compliance and quality of life in diabetes management. Further clinical trials are necessary to confirm its efficacy and safety in human subjects. The viscosity, pH, and drug content of the insulin gel were assessed physiochemically after it was made with biocompatible polymers. Franz diffusion cells were used in in vitro research to measure the gel's skin penetration, while diabetic animal models were used in in vivo investigations to gauge the gel's effectiveness in decreasing blood glucose levels. The insulin gel had favourable physicochemical characteristics and a consistent drug release profile, according to the results. The transdermal delivery of insulin was found to be effective in the in vitro permeation investigations, and the in vivo studies showed a significant reduction in blood glucose levels that was similar to that of traditional insulin injections.