Aim: This study aimed to formulate and evaluate a herbal hair care shampoo incorporating papaya seed extract. Objectives: The objectives included quantifying the extract's active constituents, assessing its antifungal activity, and evaluating various shampoo formulations. Results and Discussion: Soxhlet extraction yielded 23% papaya seed extract, with 46 grams obtained from 200 grams of seeds, demonstrating efficient bioactive compound extraction. The extract contained 34.48 mg QE/g flavonoids, exhibited 78.01% DPPH radical scavenging activity, and showed significant antifungal effects against Candida albicans, with inhibition zones of 12.8 mm at 500 mg and 13.5 mm at 1000 mg. FTIR and HPLC analyses confirmed the presence and stability of quercetin. Six shampoo formulations were developed and evaluated, all formulations displaying satisfactory pH (6-7), solids content (18-27%), and pseudoplastic viscosity. Among these, formulation F6 exhibited superior viscosity, foam stability, and antifungal activity, with a 15.8 mm inhibition zone, making it the most promising for further development and commercial production. Conclusion: Overall, the study highlighted the potential of papaya seed extract to provide significant antioxidant and antifungal properties, identifying formulation F6 as the best candidate for further development and commercialization.