The aim of present work is to formulate, evaluate and optimize microsponges loaded with isoconazole and the release of proposed formulation is controlled, which is further loaded in the form of gel.Using the quasi-emulsion solvent diffusion method, the microsponges were successfully synthesized with varying ratios of PVA as the surfactant and Eudragit RL100 as the polymer by using Box behnken design. The study looked at how the drug-to-polymer ratio, surfactant concentration, inner phase volume, and stirring speed affected the microsponges' physical characteristics and drug entrapment effectiveness. Furthermore, FT-IR methods were used to examine the drug's compatibility with different additives. The microsponges had a spherical, spongy shape, according to SEM examination. The particle sizes of the improved formulations ranged from 20.11 to 29.4 μm, and their drug entrapment efficiencies were between 73% and 97.6%. These formulations for microsponge gels were made with carbopol 934, and their pH, viscosity, and in-vitro drug release were examined.