The golden crown beard or Verbesina encelioides belongs to the family Asteraceae. It is a rich source of various phyto-constituents such as terpenoids, phenols, saponins, tannins, proteins, amino acids, anthraquinones, alkaloids, and reducing sugars etc. The plant has showed antimicrobial, antiviral, anti-tumor and anti inflammatory activities. This plant is ethnobotanically important and used in treatment of several diseases. In the present study, extracts of dried powdered V. encelioides leaves were prepared in different solvents (water, methanol and petroleum ether) and analysed for their anti-inflammatory and antimicrobial activity against selected pathogens. Water proved out to be the best solvent for extraction, though antibacterial activity was observed in methanolic extract with highest activity displayed against P. aeruginosa (AI=0.519 at 100mg/ml) and E.coli (AI=0.406 at 100mg/ml) followed by S. aureus (AI=0.316 at 100mg/ml) and B. subtilis (AI=0.366 at 100mg/ml). Similarly, the percent anti inflammatory activity was highest in methanolic extract at 20, 40, 60, 80 and 100 mg/L concentrations was 5.17, 7.42, 10.28, 15.93, and 18.28 respectively. GC-MS results discovered presence of 43 phyto-constituents in methanolic extract such as 1-Methylene 5.alpha.-androstan-3.alpha.-ol-17; n-Hexadecanoic acid, phytol, diethyl phthalate, tetradecanoic acid, 9,12-Octadecadienoic acid (Z,Z)- were among the most abundant in methanolic extract.