Acinetobacter baumanni isone of the hospital acquired infections causing serious public health challenge in Nigeria. The present study was to determine the prevalence and antibiogram pattern of Acinetobacter baumanni from two public healthcare facilities Ebonyi State. A total of three hundred clinical samples were collected analyzed using standard microbiological procedures. All suspected bacterial isolates were phenotypically screened and positive results were subjected to antibiotic resistance test using the Kirby–Bauer disc diffusion technique and Multiple Antibiotic Resistance Index (MARI). Suspected A. baumannii isolates were finally confirmed using 16S rRNA sequencing.Of all the three hundred clinical samples studied, 20(9.0 %) samples from AE-FUTHA and 1 (1.25 %) sample from Mater Misericordia Hospital were infected with A. baumannii. Catheter urine(16 %) from AE-FUTHA and (2 %) from Mater was the commonest sample sites for isolation of A. baumannii pathogen followed by wound sores (9 %) from AE-FUTHA. A .baumannii strains showed the highest level of resistance against tetracycline (100 %), trimethoprim sulphamethoxazole, (100 %), ceftriaxone (80 %) and amikacin (80 %), and lowest resistace was against meropenem (14.2 %), imipenem (19.0 %) followed polymycin B (33.3 %), antibiotics level. The result of Multiple Antibiotic Index (MARI) showed a total 12.1 and an average of 0.57. Early diagnosis of infection caused by A. baumannii and its treatment with meropenem, imipenem or polymyxin B can reduce the mortality and morbidity risks of A. baumannii infection in critically ill patients.