Green synthesis of nanoparticles can be an important alternative compared to conventional physiochemical synthesis. Terminalia chebula seed aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (ZnO NPs). Further, the synthesized ZnO NPs were subjected to various characterization techniques for their functional group and particle size as 37nm. Larvicidal activity of green synthesized ZnO NPs were tested against Anopheles third instar larvae showed significant LC50 value of 85ppm with regression coefficient R2 as 0.91. Our study concluded that T. chebula seed mediated ZnO NPs showed an effective larvicidal activity against malarial vector Anopheles third instar.