This study focused on synthesizing tin dioxide (SnO2) nanoparticles using extracts from Sugarcane leaves (Saccharum officinarum) of Poaceae family. The aim is to use green methods by using plant materials to create the nanoparticles, as opposed to chemical methods that can be hazardous. Tin dioxide nanoparticles (SnO2 NPs) are considered essential and interesting nanomaterial, and this study aimed to use agricultural waste to produce them. XRD, SEM with EDX, TEM and were used to characterize the nanoparticles. XRD spectrum shows the crystalline nature of SnO2 nanoparticle. The presences of functional groups in the nanoparticle are confirmed by FT-IR analysis. SEM and TEM images illustrate the spherical shape of nanoparticle. Synthesized SnO2 nanoparticles were evaluated for cytotoxicity and antioxidant properties. The study revealed that, SnO2 nanoparticle shows remarkable cytotoxic and antioxidant activity.