Curcumin and anthocyanins are natural compounds with potent anti-inflammatory properties. This study aims to evaluate the anti-inflammatory efficacy of a Curcumin-Anthocyanin photosensitizer gel in an in vitro setting. Methodology: The study utilized an in vitro model to assess the anti-inflammatory properties of the Curcumin-Anthocyanin photosensitizer. Anthocyanin was extracted from Punica Granatum, and Curcumin was derived from Curcuma Longa species. These extracts were combined to formulate the photosensitizer gel. The anti-inflammatory activity was measured using the BSA (bovine serum albumin) assay using Diclofenac as a control group. Results: The Curcumin-Anthocyanin photosensitizer gel demonstrated significant anti-inflammatory activity in the in vitro models. The BSA assay results indicated a notable decrease in denaturation rates, suggesting effective inhibition of inflammatory mediators in the combination group of about 53%. Conclusion: The findings from this study highlight the promising anti-inflammatory effects of the Curcumin-Anthocyanin photosensitizer gel. Its ability to reduce inflammatory mediators and exhibit beneficial photophysical properties, positions it as a potential therapeutic agent for treating inflammatory diseases and as a candidate for further research in photodynamic therapy applications.