ntroduction Atopic asthma is a chronic airway inflammation begins with exposure to allergens causing activation of Th2 cells airway hyperresponsiveness. Interleukin-33 and ST2 have role in activation of Th2 cells and then cause chronic airway inflammation. IL33/ST2 ratio considered as a potential biomarker for predicting risk and asthma severity. Aims & Methods This case-control study aimed to evaluate serum levels of IL-33, ST2 dan IL- 33/ST2 ratio in risk and level of asthma control. This study was performed in Dr. Wahidin Sudirohusodo and Hasanuddin University Hospitals Makassar Indonesia from June until September 2023. Subjects were 40 atopic asthma patients and 40 non-asthma. Result Serum levels of IL-33 higher in atopic asthma patients compared to non- asthma (1.776 + 0.534 vs 1.505 + 0.301, p=0.007), but ST2 did not significantly different. Serum levels of ST2 higher in uncontrolled atopic asthma patients compared to well controlled (9.541 + 11.634 vs 16.675 + 6.327, p=0.021), but IL-33 were not different (p>0.05). IL-33/ST2 ratio higher in uncontrolled atopic asthma patients compared to well controlled (0.266 + 0.105 vs 0.127 + 0.086, p=0.001). Conclution IL-33/ST2 ratio and levels of ST2 were significantly higher in uncontrolled atopic asthma patients compared to well controlled. IL-33 significantly higher in atopic asthma compared to non-asthma.