Human papillomavirus (HPV) infection is the most important event in malignant transformation of human cervical epithelium. The objective of this study was to develop a method allowing the detection and genotyping, quantification of HPV DNA to improve future strategies for cervical cancer screening. DNA was extracted from 47 cervical samples, then was sent to the Laboratory of Cellular and Molecular Biology, CHU Jean Minjoz, Besançon France. HPV were first detected by MY09/11 consensus PCR and then genotyped with INNO-LIPA, viral load and integration status of HPV16 was determined by real time PCR. The prevalence of HPV infection was estimated as 27.65%, the most common genotypes found were HPV 16 and HPV 52 at 38.46% and 15.38%, respectively. The increase in viral load and integrated HPV type 16 was significantly associated with CIN2, indicating that viral load and physical condition can potentially be useful triage markers for HPV16 positive women during cervical screening