Changes in weather patterns significantly contribute in dissemination of Gastrointestinal infectious(GI) disease and have been revealed to be one of the critical drivers of the emergence, re-emergence and transmission of infectious disease. This objective of the present study was to assess the impact of seasonal weather variation on three GI bacterial pathogens isolated from catfish (Siluriformes) in Abakaliki, Ebonyi State, Nigeria. A total of 58 Samples were collected, 29 dry season catfish and 29 raining season catfish. (2 samples were collected from each location) and analyzed. Bacterial isolation and characterization were conducted using standard microbiological procedures. The mean bacterial load was higher in rainy season samples (7.84 × 10⁵ cfu/g) compared to dry season samples (4.33 × 10⁵ cfu/g). Three pathogens Escherichia coli, Vibrio cholerae, and Salmonella species were identified and characterized through physiological and biochemical tests. In rainy season samples, 118 isolates were recovered: E. coli (44.9%), V. cholerae (23.7%), and Salmonella spp. (31.4%). In contrast, 96 isolates were obtained during the dry season, comprising E. coli (47.9%), V. cholerae (20.8%), and Salmonella spp. (31.3%). Antibiotic susceptibility testing revealed multidrug resistance across isolates. All E. coli strains showed 100% resistance to more than seven antibiotics, including cefepime, cefotaxime, cefoxitin, imipenem, vancomycin, nalidixic acid, and amoxicillin/clavulanic acid, but remained highly susceptibility testing revealed multidrug resistance across isolates. All E. coli strains showed 100% resistance to more than seven antibiotics, including cefepime, cefotaxime, cefoxitin, imipenem, vancomycin, nalidixic acid, and amoxicillin/clavulanic acid, but remained highly susceptible to ciprofloxacin (79%), trimethoprim (67.5%), and gentamicin (64.5%). Similarly, V. cholerae and Salmonella spp. exhibited complete resistance to more than eight antibiotics but demonstrated susceptibility to ciprofloxacin (90%) and trimethoprim (55%).The findings suggest that rising temperatures, poor sanitation, and faecal contamination in aquaculture environments contribute to increased pathogen prevalence and resistance. Given the risks associated with climate change, continuous monitoring of bacterial pathogens in fish habitats is essential. Enhanced antibiotic resistance surveillance in aquatic environments is also necessary to safeguard public health and ensure food safety.