Over the past few years, a great deal of scientific research has focused on the quest for naturally occurring antimicrobials found in plants. This study aimed to evaluate the Lemmon gras essential oil's in vitro antibacterial activity and in silico toxicity against strains of bacteria. First, the essential oil was dissolved in a solution of tween 80 and dimethyl sulfoxide (DMSO). The antibacterial action was evaluated using the minimum bactericidal concentration (MBC), which was determined by the depletion in nutrient agar ( NA) technique with aliquots of 10µL of the MIC, MIC × 2, and MIC × 4. The minimum inhibitory concentration (MIC) was determined from the microdilution in double concentrated brain heart infusion broth (BHI). In Only the Citronellol, Geraniol, and Citral fr actions showed a hazardous potential in the in silico toxicology investigation. Nonetheless, there was no indication of toxicity in the Citral fraction. Consequently, in comparison to other substances frequently used in the treatment of bacterial infections, lemon grass essential oil exhibits adequate antibacterial activity and a low potential oral toxicity.