The present study aimed to investigate the in vitro anti-diabetic and antimitotic activity of leaves extract of Dracaena trifasciata. As it was reported that Dracaena trifasciata contain flavonoids and other phytoconstituents, an attempt was made to determine its anti-diabetic and antimitotic potential. Methods The in-vitro α–amylase inhibition and antimitotic activity of the leaves extract of Dracaena trifasciata were evaluated by using 3,5-dinitrosalicylic acid (DNSA) and Allium cepa root meristematic cells. Results The result of α–amylase enzyme inhibition activity was found in a dose-dependent way, the strongest activity was shown by 25mg/ml hydroalcoholic extract 32.48±0.76%. The antimitotic activity was screened by using Allium cepa root meristematic cells. Methotrexate (0.1 mg/mL) was used as a standard. It is found that all concentration of extracts showed antimitotic activity, but 100mg/ml posses’ maximum antimitotic potential. Extract shows 61.88% mitotic index at 100mg/ml concentration.