Enydrafluctuans Lour is a medicinal flowering plant found in the Asian and African subtropics and tropics. Plant tissue culture is an important technique through which plant regeneration, mass propagation, and conservation are possible. The present study aims to standardise in vitro mass propagation technique, study of comparative antioxidant properties, and bioactive compound analysis in wild and tissue-cultured plant extracts of E.fluctuans. The explant surface sterilisation for tissue culture was best with 0.1% mercuric chloride for a 3-minute treatment, resulting in an 80% explant survival rate. In the RAPD assay, the tissue-cultured plants showed polymorphism in DNA bands by two RAPD primers. Antioxidant tests for total phenol, flavonoid contents, and total antioxidant capacity tests showed the tissue-cultured extracts carried a higher concentration of GAE (gallic acid equivalent) and QE (quercetin equivalent) comparatively than those of the wild plant. The HPLC analysis revealed that the tissue cultured extracts carried higher concentrations of gallic acid (10.1934μg/mg) than in the wild plant extract (8.059μg/mg) and higher concentration of quercetin ( 5.1292μg/mg of dried extract) than the wild plant extracts (2.0199μg/mg). The bioactive compounds in the extracts of wild and tissue-cultured plants were studied using GC-MS analysis.