Background: Papaya (Carica papaya L.) is a significant commercial fruit crop in the tropics, and successful micropropagation is crucial for the mass production of superior true hermaphrodite genotypes such as 'Tainung No. 2'. Objective: This study aimed to establish an effective protocol for rapid in vitro regeneration and clonal multiplication from nodal explants of hermaphrodite papaya 'Tainung No.2'. Methodology: Nodes used for explants were cultured on MS medium containing hormone BAP (0-1.0 mg L-1) along with Kinetin (10 mg L-1) or NAA (0.1-0.5 mg L-1) for shoot formation. Multiplication and elongation were determined by observing the response of BAP, NAA, and casein hydrolysate (CH) at varying concentrations (000–500 mg l⁻¹). Results: The maximum value of shoot induction was recorded as 66.67 %, using 0.5 or 1.0 mg l⁻¹ BAP and 0.1 mg l⁻¹ NAA. Maximum multiplication and elongation had been observed when 0.5 mg l⁻¹ IBA, 0.2 mg l⁻¹ NAA, and 200-300mg l⁻¹ CH were used. Rooting was successful in a half-strength MS basal medium containing 1.5 mg L⁻¹ IBA and 200 mg L⁻¹ CH. Conclusion: The developed protocol enables a high rate of clonal propagation of papaya 'Tainung No.2' and successful acclimatization of the plantlets.