Ocimumcanum, known as Kali tulsi, is a versatile medicinal plant abundant in phytochemicals, offering a spectrum of therapeutic applications for various ailments. It has been extensively studied for its phytochemical composition and antimicrobial traits. Particularly focusing on locally available strains in the Chhattisgarh region, an investigation has been undertaken. Results garnered significant scientific attention confirming important beneficial contents inherent in the plant extracts. High-Performance Thin-Layer Chromatography (HPTLC) analysis unveiled distinct compounds with Rf values at 0.099 and 0.183, exhibited the presence of anti-oxidants, while Fourier Transform Infrared (FTIR) analysis revealed the presence of intricate organic compounds like aldehydes, phenols, and carboxylic groups. Antioxidant evaluations using DPPH scavenging assays revealed strong antioxidant activity, which can be attributed to the significant phenolic content present in the plant extracts.Total phenol content determination provided valuable insights into the chemical makeup of the plant. Experimental assays demonstrated potent antimicrobial effects against pathogenic bacteria and fungi, highlighting its therapeutic significance.