Background Various in vivo and in vitro studies have found the effects of herbo minerals in treating cancers and cervical cancer being the 4th commonly occurring cancer in women. The combined anticancer, anti-oxidant effects of herbs, minerals and heavy metals provide us a scope for treating cancer patients effectively. In this study we studied the effect of Panchapadana Chenduram on HeLa (cervical adenocarcinoma) cell lines at different concentrations. Objective To observe the level of anticancer and antiproliferation capacity of Panchapadana Chenduram on HeLa (cervical adenocarcinoma) cell lines. Materials and methods The HeLa (cervical adenocarcinoma) cell lines were treated with different concentration of 10, 50,100,150 and 200µg/ml. The viability of the cells is checked, percentage growth inhibition using MTT assay. Results At varying concentrations, significant changes were observed in the cell viability, antiproliferative capacity of the test drug. High percentage of cell death was observed at the concentration of 200µg/ml 35.65 ± 1.606 % and IC50 value of the sample was found to be 276.1 ± 22.12 µg/ml evidencing that the test drug PPM possess convincing anti-cancer activity. Conclusion The results of the current study point towards the usage of Panchapadana Chenduram as an efficient and safe chemotherapeutic agent in future, with more evidences required on HeLa (cervical adenocarcinoma) cell lines to treat cervical cancer.