Diabetic neuropathic pain is a prevalent, severe, and incapacitating condition that poses a huge obstacle to medical care. There are numerous medications on the market, but no effective conventional treatments exist for neuropathic pain. In medical research, herbal formulation has received increased interest lately. Specioside is one of the isolated compound from African traditional medicines is Kigelia africana which exhibits a wide range of therapeutic value and has a lot of scientific interest due to its wide range of pharmacological properties to treat illnesses. Additionally, phytoconstituents from the plant are known to exist, that was found in n-hexane, chloroform, ethanol, and ethyl acetate aqueous extracts including alkaloids, glycosides, phenols, flavonoids, and other phytoconstituent are known as speciocide.This speciocide emerging antidiabetic effect interconnection with diabetic secondary complications of diabetic neuropathy, retinopathy, and nephropathy. Our study mainly focused on the Invitro inhibitory assay of enzyme α-amylase hydrolyzing the glycosidic linkages in carbohydrates to produce oligosaccharides, which are broken down