As agriculture is main occupation in India which is not only affected by natural calamities such as draughts and floods but also by disease causing microorganisms. To control the damage caused by pathogenic microorganisms, previously chemicals were used but now microbes are widely used as biocontrol agents.Out of various groups of microbes, some of the microbial communities will offer a wide range of securities to the host plant includes pathogen avoidance,pests and insects repelling and growth promotion.Therefore, in present study we investigated on microbiome of agricultural soils.Soil samples were collected from different fields like Paddy, Cotton, Maize, Red gram and Mirchi. A number of actinomycetes isolates were obtained from various soil samples.The isolates were identified based on the morphological study, microscopic observation and biochemical profile. Antimicrobial activity is carried out by several test organisms:Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 25922), Klebsiellapneumonia(ATCC 700603), Pseudomonas aeruginosa (ATCC 27853). The isolated actinomycetes were further inoculated on Pikovskaya’s agar media and on Carboxy Methyl Cellulose agar media to study their phosphate solubilizing and cellulolytic activity.