This study evaluates the properties and stability of 2-hydroxy-1,4-naphthoquinone and its gel formulations (F-1, F-2, and F-3) for pharmaceutical applications. The compound, characterized by a dark reddish-brown crystalline form, earthy aroma, and bitter taste, demonstrates solubility in various solvents, including ethanol, methanol, and acetone. Gel formulations were assessed for flow properties, pH, consistency, homogeneity, spreadability, and viscosity. Results indicate that F-1 exhibits superior flowability and optimal application characteristics, followed by F-2 and F-3. All formulations are stable over time and suitable for topical use, with slight variations in texture and pH. The findings highlight the importance of formulation characteristics in ensuring stability and efficacy for dermatological applications.