India is experiencing a conjunctivitis outbreak, affecting many people and causing economic and social burdens. Knowledge, attitude, and precautionary steps are crucial for population education. The study aims to evaluate conjunctivitis knowledge and attitudes in rural areas of Salem district, identify preventive measures, source of information, and treatment strategies used by affected individuals. The cross-sectional observation study was conducted for six months among 200 rural respondents. The respondents were selected by considering the inclusion and exclusion criteria. A survey was conducted to assess awareness and knowledge on Conjunctivitis spread, symptoms, treatment, vector characteristics, preventive measures, and sources of information based on socio demographic details and questionnaires. Among the 200 respondents, the duration of recovered respondent was found to be up to 6 months (41.0%) for majority of the respondents. The Type of Treatment shows that majority of the respondents were home isolated (50.0%) and some of the respondents visited the government hospital (38.5%). Most of the residents (70.8%) gave wrong answers for the questions asked to assess the knowledge about conjunctivitis. Most of the residents (46.2%) agreed to the questions asked to assess the attitude towards conjunctivitis. Many number of residents (64.3%) gave a correct responses for the questions asked to evaluate the practices regarding conjunctivitis. The study found that many residents have lack of knowledge and attitude towards conjunctivitis, suggesting the need for increased awareness programs on signs, symptoms, and self-hygienic care.