Background: Malignant tumors of the head and neck represent 6,6% of all tumors registered by the American Cancer Society, with laryngeal cancer accounting for almost 21% of the head and neck region. The development of surgical techniques that preserve function, as well as improving or maintaining survival, has been a priority in laryngeal, as in other types of cancer, for the last 50 years. After the introduction of the CO2 laser for the treatment of benign and very early malignant laryngeal lesions by Strong and Jako . Endoscopic Transoral Laser Microsurgery permits complete tumour resection, yet is associated with higher rates of laryngeal preservation, less morbidity (particularly swallowing defects) lower frequency of pharyngocutaneous fistula and shorter hospitalization than open surgery, it is possible to minimize the loss of healthy tissue and to lower the overall morbidity. Hence the use of transoral laser surgery has been internationally accepted as a valid treatment option for early laryngeal cancer; however, its use in advanced laryngeal diseases remains controversial Aim: This study as held to investigate the possibility for proper resection of selected cases of laryngeal cancer using trans-oral Laser Microsurgery as an alternative to total and partial laryngectomy Methodology: 40 patients diagnosed as Laryngeal Cancer and indicated for partial or total laryngectomy were enrolled in this study. after removal of the specimen it will be fixed on a special holder and the tumor will be removed by laser with safety margin. The two specimens (the resected larynx and laser excised tumor) will be subjected to detailed histopathological examination to detect the presence of tumor in the remaining part of the larynx after laser excision of tumor and in the safety margin around the laser excised tumor. The safety margin around the laser excised tumor will be measured. Results: Positive margin was found at surgical upper(A) margin (22.5%), surgical lower (D) margin (12.5%). However, at surgical lateral (B– C) margin no positive cases were reported, and higher positive margin was found among laser upper (A`) margin (32.5%),laser lateral (B` – C`) margin (17.5%) and finally laser lower(D`) margin (47.5%) of all studied patients. Regarding tumor type: the cases with both sites’ lesions had higher positive margin, followed by supraglottic (40%) lesions in A than glottic lesions (0%). However, in D` margin glottic lesions (31%) had higher positive margin than supraglottic lesions (0%) Conclusion: Transoral laser microsurgery is a suitable alternative to total or partial laryngectomy with statistically significant agreement for margins between both techniques