In West Bengal, India's Murshidabad region, indigenous tribes have been using medicinal trees for centuries. This research work aims to document and analyse their traditional knowledge of these species. The goal of the study is to identify and describe the therapeutic uses of trees in traditional healthcare systems. Observations in the field, surveys, and interviews were a few of the qualitative and quantitative research methods employed. The survey found 79 different kinds of medicinal trees in the area, representing a wide range of taxonomic groups. The local populations have developed great knowledge about these trees and how to use them to treat a variety of illnesses and health issues. Interviews with traditional healers and informed community members provided important details about the indigenous usage, preparation techniques, and dosage of medicinal tree parts. The results showed that the traditional users in the Murshidabad district heavily depend on the bark, leaves, roots, flowers and fruits of medicinal trees for therapeutic purposes. The digestive, respiratory, skin, and febrile illnesses that were most commonly mentioned as being cured by these trees. The traditional healers also demonstrated a thorough awareness of the characteristics, particular applications, and potential negative consequences of the trees. The study also emphasised the significance of keeping traditional knowledge about medicinal plants because it offers a sustainable and affordable form of healthcare for regional people. Further scientific research is required to confirm the efficacy and security of these folk treatments, which is another point that is made here.