Objectives- We studied the effect of a single bout of mindfulness meditation on cardiovascular reactivity (CVR) to acute mental stress to devise a method to assess and attenuate mental stress among Indian undergraduate medical students. Material and Methods- This was a pre- and post-comparative interventional study conducted in thirty healthy male under- graduate medical students. Anthropometric measurements, Heart rate and Blood pressure readings were recorded during the assessment. These healthy male undergraduate medical students were selected based on age criteria, history of prior exposure to mindfulness meditation. Data of Pre-mindfulness meditation reactivity (T1-R1) and Post mindfulness meditation reactivity (T2-R2) were compared and analysed for significance to draw a conclusion. Results- Systolic blood pressure reactivity when compared between Pre Mindfulness Meditation reactivity (T1-R1) and Post Mindfulness Meditation reactivity (T2-R2) showed statistically significant results with p value 0.01, with no significance seen between diastolic blood pressure reactivity and Heart rate reactivity. Conclusion - A significant difference in systolic blood pressure reactivity after mindfulness meditation, points towards the role of mindfulness meditation in decreasing sympathetic flow as opposed to its increased sympathetic response seen during mental stress without mindfulness meditation intervention