It is well established in the literature that mindfulness meditation helps in reducing metabolic and psychological stress, thus improving well-being. The present study aims at investigating this beneficial effect of mindfulness meditation in the context of the Islamic Pilgrimage (El-Hajj), through an oxidative and metabolic approach. Our experimental group includes 30 volunteer subjects who regularly practiced mindfulness meditation through Islamic prayer. The control group includes 30 subjects who do not practice prayer. Those who practice prayer (hajjis) are sampled one week prior to their departure to the pilgrimage and one week after their return from it. Stress parameters include Malondialdehyde (MDA), Catalase (CAT), and Cortisol. Metabolic parameters include control of fasting blood glucose, triglycerides, total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL). Our results show a significant reduction in plasma cortisol levels (p=0.014) in the hajjis after return from the pilgrimage. This decrease is associated with a decrease in lipid peroxidation levels (p = 0.082) and a significant increase in antioxidant defense by catalase (p = 0.0466). We suggest that mindfulness meditation through Islamic pilgrimage and prayer accounts for a downward modulation of plasma cortisol, MDA and the activation of antioxidant defense by catalase in hajjis after pilgrimage.