Background: DNA fingerprinting is a simple technique is used to identification of nucleotide sequences of the medicinal plants and DNA sequences. Aim and Objectives: Molecular Authentication of Crinum Solapurense Plant by Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD) -Barcoding Technique. The main objective of this research work was to identification of Particular DNA sequencing from group of unknown and protection of biodiversity. Results and Discussion: We used AP l, AP4. AP11 and OPAB 11 decemer primers to identify the sequencing the genetic variation of ayurvedic medicinal plants. Conclusion: The DNA was amplified with four decemer primers and polymerase chain reaction primers, and then analyzed on 1 % agarose gels.