ISSN : 2663-2187

Motility, viability and morphological defects in fresh and liquid preserved semen of indigenous Bengal sheep

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Md. Manik Mia, Mohammad Mahbubul, Tahrim Sumaiya, Md. Younus Ali, Auvijit Saha Apu
» doi: 10.48047/AFJBS.6.15.2024.14174-14195

Abstract

Indigenous Bengal sheep is a potential native germplasm of Bangladesh. Evaluation of semen quality is essential to preserve the semen for future use. This study focuses the fresh and short term preserved (at 4°C) liquid semen quality of indigenous Bengal sheep. Thirty ejaculates were evaluated, then diluted using egg yolk-citrate diluter and preserved for 5 days at 4°C.Fresh semen parameters e.g. volume (ml), concentration (× 106), progressive motile (%), and live spermatozoa (%) were 0.70±0.04, 4812.00±92.05, 72.63±0.58, and 80.18±0.55, respectively. Total sperm motility was significantly (p<0.05) highest (80.42±0.97%) at Day 0 while lowest (52.67±2.47%) at Day 5 during preservation. Progressive motility percentage was found to be the best at Day 0 (69.93±0.32%) followed by Day 1 (64.75±0.45%), Day 2 (59.87±0.86%), Day 3 (52.58±0.64%), Day 4 (40.09±3.27%) and Day 5 (31.12±2.96%) whereas oscillatory and rotatory motility percentage showed opposite trend. Viable spermatozoa percentage significantly (p<0.05) dropped gradually from 71.63±0.76 to 32.74±1.78%, while morphological defects (head, mid piece and tail) increased with preservation time, highest at Day 5 and lowest at Day 0. The quality of Bengal sheep semen declined as the liquid preservation at 4°C duration was extended, and after three days, progressive motility retention was more than 50%.

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