Background: A vital component in cellular bioenergetics and adaptive responses to stress is NAD. NAD deficiency is linked to age-related illnesses, malignancy, and neurological disorders, it has been the focus of numerous studies. NNM is an orally accessible precursor of NAD that has been shown to have positive impacts on the body's levels of NAD, which helps prevent aging and several disorders. Thus, to assess NMN's potential as a treatment, we carried out the study to determine its role in increasing NAD levels.