Consistent evidence in the literature indicates a correlation between ABO blood group and type 2 diabetes mellitus. Numerous studies have established a correlation between particular genetic variants and an elevated risk of type 2 diabetes mellitus, one of which involves a polymorphism in the KCNJ11 gene. This study aims to analyze the prevalence of KCNJ11 gene variants in relation to blood type in Bengkulu. This study is a descriptive observational research employing a cross-sectional design. The present study analyzed the link between blood groups and KCNJ11 gene polymorphisms. The correlation between variables was examined with the Chi-Square Test. This study's results indicated no significant association between ABO blood group and KNCJ11 gene polymorphisms (p=0.232; p=0.825; p>0.05). Blood type analysis indicated that participants with a history of F1 in T2DM patients predominantly exhibited the EE genotype in the O group (57.1%), whereas the EK+KK genotype was prevalent in the non-O group (66.7%). The group in the F1 of Non-T2DM patients was predominantly composed of EK+KK in the non-O group (54.5%). The EE genotype in individuals with blood type O may elevate the risk of diabetes.