Our work aims to optimize the extraction conditions of poly phenols from cypress leaves (Cupressus sempervirens L.) by maceration in methanol and distilled water v/v 70% / 30% respectively, after stirring for 24 hours, we proceeded to filtration, a dry evaporation was carried out in order to obtain the dry extracts to be studied. The results helped to determine the optimal conditions for maximizing the total poly phenol content of extracts. Optimal extraction was achieved with 100 μg EAG/mg DM and 160 μg EAC/mg DM flavonoid. We obtained with a powder concentration of 21.17% and an extraction time of 24 hours. The anti-radical activity demonstrated a strong inhibition of DPPH thanks to the richness of the extract in secondary metabolites. The HPLC separation chromatographic method used in our work identified 6 secondary metabolites: gallic acid, tannic acid, vanillin, quercetin, Rutin and salicylic acid.and we obtained the major flavonoid that is rutin with a maximum concentration of 8.23 0.01 mg/g MS