The Topical-delivery possessions of the new prospective nano-carriers drug liberation systems, which were accustomed increase the time retention on the anticancer agent in the topically body. In this drug distribution systems occurred extensively exploited to distribute anticancer drugs. The 5-fluorouracil entrapped soya-lacithin30% transferosomal preparation in this broad-fields have the great beneficial effect. Now, we obtained that while the rational quantity of 5-fluorouracil and soya-lacithin 30% was 1:1. Results showed that the average particle size, polydispersity index, and zeta potential were 35.58±0.56, 0.285±0.125, and 14.50 ±0.8 respectively, for 5FU loaded transfersomes. And image shown that TEM, FTIR Study, X-ray diffraction study mention below. The highest encapsulation efficiency achieved was 85.05 ± 0.58 for P-16 formulation