The contact between host plants and pathogens induces the production of phytoalexins composed of phenols, to resist against the pathogen. The aim of this study is to highlight this phenomenon using in vitro culture, and select resistant genotypes of chickpea (Cicer arietinum). Calluses of Cicer arietinum are exposed to the filtered filtrate (enzymes and toxins) of Ascochyta rabiei to demonstrate the production of phenols in the process of resistance. The calluses are produced from INRA 199 and FLIP 84-92C genotypes, as well as the Twist genotype of C. arietinum. The hormonal balances used are: K1 (3 mg/l 2,4-D and 1 mg/l BAP), K2 (2 mg/l 2,4 D), Ma (2 mg/l 2,4-D and 4 mg/l Kinetin), and M8 (2 mg/l 2,4-D, 4 mg/l NAA, and 1 mg/l Kinetin). The calluses are confronted with the filtered filtrate from isolates H1 and E2 of A. rabiei. Phenol extraction is carried out under reflux, and their quantitative estimation is performed using spectrophotometry. The results of callogenesis are influenced by genotype, organ, and hormonal balance (nature and concentration). Significant callogenesis from all four genotypes is recorded on medium K1. The results related to the interaction between C. arietinum calluses and enzymes and toxins of A. rabiei indicate that the highest quantities of phenols are produced by INRA 199 genotypes.