Silver prices are rising along with GDP and market. Silver being the most expensive metal was used for many applications, so, it has been quite difficult for the resources of silver to meet its demand. Instead of depleting the resources, research took turn towards search for the possible reuse of available sources. X-rays waste (Used photographic films) was considered as major source for silver recovery in the present research. Recovery was done using Bromelain enzyme extracted from waste Musa Acuminata peel waste. Utilizing the optimized conditions and making use of Bromelain enzyme Silver is recovered in nano scale which has a wide variety of applications in various sectors. Silver recovery was optimized by Response Surface Methodology, experimentation revealed that at a pH of 9, considering 15mL of Bromelain enzyme, by agitating for 40min at 80℃ from 0.6g of X-rays provided optimum silver recovery from photographic films. Characterization was performed from which nano sized silver particles were confirmed from SEM, XRD, surface area was analysed using BET, TGA, FTIR were performed for Silver extracted from Musa Acuminata peel waste