This study aimed to pharmacologically evaluate the potential anti-urolithic activity of rain lily (Zephyranthes spp.) plant extract in an experimental rat model. Method: The rain lily plant extract was obtained and subjected to phytochemical screening to identify its constituents. Male Wistar rats were divided into groups and induced with urolithiasis usinga standard protocol. The rats were then administered varying doses of the rain lily extract orallyfor a specified duration. Parameters such as urinary pH, urinary volume, calcium oxalate deposition, and serum biochemical markers were assessed to evaluate the anti-urolithic activity of the extract. Result: Phytochemical screening of the rain lily extract revealed the presence of alkaloids, flavonoids, and glycosides, among other constituents. Treatment with the rain lily extract significantly increased urinary pH and volume while reducing calcium oxalate crystal deposition in the kidneys of urolithiasis-induced rats. Moreover, serum levels of biochemical markers associated with kidney function were restored towards normal levels in the treated groups compared to the control. Conclusion: The findings of this study indicate that the rain