Pituranthos scoparius is a plant that grows annually across various areas in Algeria, and has traditionally been used in folk medicine. This study aims at determining the phenolic profile, antioxidant, and enzyme activities of ethyl acetate extractsfrom the aerial parts of Pituranthos scoparius collected at three different phenological stages(vegetative, flowering, and fruiting). Antioxidant activity was assessed using β-carotene–linoleic acid, DPPH•, ABTS•+, CUPRAC, and metal-chelating assays. For the first time, the enzyme inhibitory potential of the tested extracts was evaluated against key enzymes implicated in neurodegenerative disorders, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), as well as urease. Additionally, the inhibitory activity of the extracts was assessed against tyrosinase. The results showed that phenological stages affected significantly P.scoparius activities. Furthermore, ethyl acetate extract at flowering stage exhibited the greatest activities.